어류 및 갑각류 중에 잔류하는 fluoroquinolone계 항균제를 조사하기 위하여 HPLC-FLD와 LC-MS를 이용하여 분석을 실시하였다. HPLC-FLD를 이용하여 분석하였을 때 검출한계와 정량한계는 각각 0.0030-0.0083, 0.0090-0.0252 mg/kg이었고, LC-MS는 각각 0.0019-0.0040, 0.0059-0.0122 mg/kg으로 나타났다. ofloxacin, norfloxacin, pefloxacin, ciprofloxacin and enrofloxacin의 회수율은 76.4-104.6% 범위로 분석되었다. 총 268건에 대하여 fluoroquinolone계 항균제의 잔류량을 조사한 결과 7.5%의 검출율을 나타내었고, 동자개 1건에서 enrofloxacin과 ciprofloxacin의 합이 허용 기준치인 0.1 mg/kg보다 높게 검출되었다. Ofloxacin, norfloxacin과 pefloxacin은 모든 시료에서 검출되지 않았고 ciprofloxacin과 enrofloxacin은 각각 3.4%와 6.7%의 검출율을 보였다. 어종별 검출율은 장어(2.6%), 미꾸라지(2.2%), 동자개(1.5%), 붕어(0.7%), 가물치(0.4%) 순서로 나타났다.
The residual contents of fluoroquinolones in fish and shrimp were analyzed by using HPLC-FLD and LC-MS. The limit of detection (LOD) and limit of quantification (LOQ) for fluoroquinolones by HPLC-FLD were 0.0030-0.0083 and 0.0090-0.0252 mg/kg, respectively. Those by LC-MS were 0.0019-0.0040 and 0.0059-0.0122 mg/kg, respectively. Recoveries regarding ofloxacin, norfloxacin, pefloxacin, ciprofloxacin and enrofloxacin ranged from 76.4-104.6%. The detection rate of fluoroquinolones was 7.5% from a total of 268 samples. In only one Korean bullhead sample, the sum of ciprofloxacin and enrofloxacin contents was detected at a higher level than its maximum residue limits (MRL) of 0.1 mg/kg. Ofloxacin, norfloxacin and pefloxacin were not detected in any samples and detection rates of ciprofloxacin and enrofloxacin were 3.4% and 6.7%, respectively. In detection rate by species eel (2.6%), loach (2.2%), Korean bullhead (1.5%), carp (0.7%) and snakehead (0.4%) were in order.
