Previously, we showed that Dangguisoo-san (DGSS), an herbal formula that has been traditionally used for the treatment of blood stagnation, is also applicable for inflammatory lung diseases. Activation of Nrf2, an anti-inflammatory transcription factor, and suppression of NF-${kappa}B$, a pro-inflammatory transcription factor, were suggested as an underlying mechanism. However, the constituents responsible for these activities remain unidentified. To this end, we prepared the water extracts of the 9 constituents of DGSS and tested for their effect on Nrf2 by using an Nrf2-Luciferase reporter cell line and western blot analysis. Results show that Carthamus tinctorius L.(CT), one of the 9 constituents of DGSS, strongly activated Nrf2. Similarly, when measured the effect of the 9 constituents on NF-${kappa}B$ by using an NF-${kappa}B$-Luciferase reporter cell line and western blotting for nuclear p65, indicative of activated NF-${kappa}B$, most constituents were capable of suppressing NF-${kappa}B$ in various degrees. However, CT and Cyperus rotundus L. (CR) strongly suppressed NF-${kappa}B$ activity elicited by LPS. Of note, CT activated Nrf2 and suppressed NF-${kappa}B$ strongly as well. Our results contributes to corroborating the anti-inflammatory effects of DGSS by identifying CT and CR as two major herbs responsible for activating Nrf2 and suppressing NF-${kappa}B$. These results suggest that CT and CR represent some of the effects of DGSS in the regulation of inflammation.