실험동물은 생후 4주령 Sprague Dawley계 수컷 흰쥐 24마리로 흑마늘운동군(6마리), 운동군(6마리), 흑마늘군(6마리), 대조군(6마리)으로 총 24마리로 구분하여 운동은 총 4주간 트레드밀로 실시하였으며, 시료 채취 하기 바로 직전에 고강도 운동을 실시하였다. 흑마늘 섭취는 흑마늘진액을 구강 투여시켰으며, 연구결과 다음과 같다. 4주간의 흑마늘 투여와 유산소운동 후 고강도 운동을 실시하였을 때 TBARS 농도는 흑마늘운동군이 흑마늘군, 운동군과 대조군에 비해 유의하게 낮았다. 흑마늘군과 운동군은 대조군에 비해 유의하게 낮았다. HSP 70 발현은 흑마늘섭취군이 다른군에 비해 상대적으로 낮게 나타났다. COX-2 발현은 모든군에서 비슷한 발현을 보이고 있으며 규칙적인 운동과 흑마늘 섭취는 산화적 스트레스를 줄일 수 있을 것으로 기대된다.
The purpose of this study was to investigate the effects of black garlic supplementation and exercise on TBARS, HSP 70, and COX-2 expression after high-intensity exercise in rats. Twenty-four male Sprague-Dawley rats (4 weeks) were used in this study. Experimental groups were a control group (A, n=6), black garlic supplementation group (B, n=6), exercise group (C, n=6), and black garlic supplementation with exercise group (D, n=6). High-intensity exercise was performed by treadmill running (every 10 min speed increased gradually; 15~24 m/min, $0^{circ}$ grade) and 2.86 g/kg black garlic for 4 weeks. The findings of this study were as follows: TBARS levels were significantly lower in the D group compared to the other group and B and C groups were significantly lower in TBARS levels than in the A group. HSP 70 expression was lower in the B group compared to the other groups. COX-2 expression has showed a similar expression in all groups. Therefore, it is considered that black garlic intake and exercise may reduce oxidative stress.
