The objectives of this study were to evaluate the physicochemical characteristics of puffer fish under storage conditions. Free amino acids were identified in the order of taurine > alanine > lysine > leucine > glutamic acid > valine. Glutamic acid, lysine, histidine, arginine, proline, and aspartic acid increased over time and with increased temperature, and valine and tyrosine were affected by temperature. ATP decreased dramatically during 36 h of storage at $4^{circ}C$, 24 h of storage at $12^{circ}C$, and 16 h of storage at $20^{circ}C$. IMP reached its highest level when puffer fish was stored for 36 h at 4 and $12^{circ}C$ and 24 h at $20^{circ}C$, and hypoxanthine levels grew steeply at 60 h at $4^{circ}C$, 24 h at $12^{circ}C$ and 20 h at $20^{circ}C$. In terms of K value, the puffer fish was available for sliced raw fish within 60 h at $4^{circ}C$, 24 h at $12^{circ}C$ and 12 h at $20^{circ}C$, and the fish can be taken in after cooking within 72 h at $4^{circ}C$ and $12^{circ}C$ and 36 h at $20^{circ}C$. The physicochemical quality characteristics showed that puffer fish is available for sliced raw fish within 36 h at $4^{circ}C$, 16 h at $12^{circ}C$ and 12 h at $20^{circ}C$, and that the fish can be taken after cooking within 72 h at $4^{circ}C$ and $12^{circ}C$ and 36 h at $20^{circ}C$.