A 2-month-old, intact female, maltese puppy was presented with an acute onset of hematuria and stranguria. The dog was diagnosed as urotliths in bladder using radiography of abdomen. Struvite uroliths in bladder were confirmed by Minnesota Urolith Center after surgical removal of uroliths on local clinic. There were 3 struvite uroliths in bladder and it caused hematuria. The dog had no problem including hematuria for 2 months after surgical removal of uroliths with preventative diet for struvite uroliths.