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Rapid Detection of Food-borne Listeria monocytogenes by Real-time Quantitative Loop-mediated Isothermal Amplification
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  • Rapid Detection of Food-borne Listeria monocytogenes by Real-time Quantitative Loop-mediated Isothermal Amplification
  • Rapid Detection of Food-borne Listeria monocytogenes by Real-time Quantitative Loop-mediated Isothermal Amplification
저자명
Shan. Xiaoxiao,Zhang. Yaoqi,Zhang. Zhigang,Chen. Miaorui,Su. Yongyu,Yuan. Yingna,Alam. M. Jahangir,Yan. He,Shi. Lei
간행물명
Food science and biotechnology
권/호정보
2012년|21권 1호|pp.101-106 (6 pages)
발행정보
한국식품과학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

The purpose of this study was to develop a real-time quantitative loop-mediated isothermal amplication (LAMP) method for the rapid, sensitive, and convenient detection of Listeria monocytogenes in food. The LAMP method could amplify the hlyA gene of L. monocytogenes successfully at $63^{circ}C$ with a loopamp real-time turbidimeter. The detection limits of the LAMP for hlyA gene were 6 colony forming units (CFU)/tube. A standard curve was generated for L. monocytogenes LAMP by plotting the graph based different log CFU values of L. monocytogenes and time of positivity through real-time monitoring of the amplication. Then, the LAMP method was employed to test 94 retail food samples effectively. Sensitivity in detection of L. monocytogenes by the LAMP was higher than that of PCR and none of the conventional method-positive samples was missed by the LAMP method.