The seismic safety of long-period cable-stayed bridges is assessed by probabilistic finite element analysis and reliability analysis under NFE. The structural response of critical members of cable-stayed bridges is evaluated using the developed probabilistic analysis algorithm. In this study, the real earthquake recording(Chi-Chi Earthquake; 1997) was selected as the input NFE earthquake for investigating response characteristics. The probabilistic response and reliability index shows the different aspect comparing the result from FFE earthquake. Therefore, the probabilistic seismic safety assessment on NFE earthquakes should be performed for the exact evaluation of long-period cable-stayed bridges and the earthquake resistant design criteria should be complemented.