MIMO-OFDM which is one of core techniques for the high-speed mobile communication system requires the efficient channel estimation method with low estimation error and computational complexity, for accurately receiving data. In this paper, we propose a channel estimation algorithm with low channel estimation error comparing with LS which is primarily employed to the MIMO-OFDM system, and with low computational complexity comparing with MMSE. The proposed algorithm estimates channel vectors based on the LMS adaptive algorithm in the time domain, and the estimated channel vector is sent to the detector after FFT. We also suggest a preamble architecture for the proposed MIMO-OFDM channel estimation algorithm. The computer simulation example is provided to illustrate the performance of the proposed algorithm.