Biodiesel is technically competitive with or offers technical advantages over conventional petroleum diesel fuel. Biodiesel is an environmentally friendly alternative liquid fuel that can be used in any diesel engine without modification. In this study, to investigate the effect of fuel injection timing on the characteristics of performance with DBF in DI diesel engine. The engine was operated at five different fuel injection timings from BTDC $6^{circ}$ to $14^{circ}$ at $2^{circ}$ intervals and four loads at engine speed of 1800rpm. As a result of experiments in a test engine, maximum cylinder pressure is increased with leading fuel injection timing. Specific fuel oil consumption is indicated the least value at BTDC $14^{circ}$ of fuel injection timing.