우리나라 고유종인 기름종개의 난발생 및 초기생활사를 연구하기 위해 2007년 6월에 전라북도 남원시 운봉읍의 낙동강에서 성숙한 개체를 채집하였다. 채집된 개체는 LHRH-1을 주사하여 건식법으로 인공수정시켰다. 성숙란은 분리침성란으로 밝은 회색이었으며, 난경은 $1.29{pm}0.07mm$였다. 수정난은 수온 $23{sim}25^{circ}C$에서 수정 후 45~52시간 사이에 부화하였으며 크기는 전장 $4.5{pm}0.24mm$였다. 부화 후 5일 후에 난황이 모두 흡수 되어 후기자어로 이행하고 전장 $6.6{pm}0.13mm$로 성장하였으며, 부화 후 25일에는 지느러미 기조가 모두 정수로 되어 치어기로 이행하였고 전장 $9.0{pm}0.49mm$로 성장하였다. 부화 후 100일 후에 반문모양과 외부형태가 성어와 유사하였고 전장 $27.3{pm}2.82mm$로 성장하였다.
The spine loach, Cobitis hankugensis endemic to Korea were collected at the Nokdong River, Unbong-eup, Namwon-si, Jeollabuk-do during June 2007 for study its eggs development and early life history. The eggs were obtained by injecting gonadotropin (LHRH-1) to female and were fertilized by dry method in the laboratory condition. The matured eggs were separative, demersal and light yellowish with $1.29{pm}0.07mm$ in diameter average. Under $23{sim}25^{circ}C$, the eggs were hatched in 45~52 hours after fertilization, and just hatching larvae were $4.5{pm}0.24mm$ in total length. On 5 days after hatching, they were $6.6{pm}0.13mm$ in total length and their yolk sacs were completely absorbed. From 25 days after hatching, they became the juvenile stage with $9.0{pm}0.49mm$ in total length. On the 100 days after hatching, their external forms and band patterns were similar to those of adults with average $27.3{pm}2.82mm$ in total length.
