Objective : This study was designed to analyze the results of autonomic nervous activity(ANA) and autonomic nervous balance(ANB) by heart rate variability test. The subjects were workers in the automobile manufacturing company. Method : The subjects consisted of 32,049 workers who had answered about questionnaires of a job position, age, smoking, drinking, exercise history and sex. The ANA and ANB were examined by SA3000P. I analyzed the differences of ANA and ANB according to job positions, ages, the state of smoking, drinking, exercise and sex by T-test or ANOVA with SPSS ver. 17.0. Results : Regarding the differences of ANA among job positions, the ANA was lowest in sales positions, highest in production workers. Among ages, the ANA was highest in 50s, lowest in 30s. In smoking, the ANA was higher in non-smoking group. In drinking, the ANA was higher in non-drinking group. In exercise, the ANA was higher in exercise group. In sex, there was no significant difference. The case of ANB, there were no significant differences except for ages. Among ages, the ANB was highest in 20s, the lowest in 40s. Conclusion : According to these results, we should establish the methods of controlling ANA and ANB from the perspective of Korean traditional medicine.