Purpose: This study examined the effects of taekwondo exercise program on the physical function, fitness and quality of life in women with osteoarthritis. Methods: With nonequivalent control group quasi-experimental design, 17 outpatients with osteoarthritis were recruited from June to August, 2010. The subjects were divided into 14 in experimental group and 13 in control group. The taekwondo exercise program was consisted of public taekwondo gymnastics with stretching movement and carried out every 60 minutes, 2 times a week for 12 weeks. Physical fitness of sit up, sit and reach, and agility, KWOMAC, and quality of life were measured. Data were analyzed by t-test and Mann-Whitney test using SPSS program. Results: After the intervention, the physical fitness for muscle endurance (Z=-4.40, p<.001), flexibility (Z=-2.75, p=.006), and agility (Z=-1.95, p=.050) were significantly improved in the experimental group. The mean scores of KWOMAC in the experimental group were significantly decreased (Z=-2.94, p=.003) and the mean difference of quality of life in the experimental group was also significantly increased than that of the control group (Z=-3.50, p<.001). Conclusion: The taekwondo exercise program may have affirmative effects on physical fitness, physical function, and quality of life in women with osteoarthritis.