The purpose of this study was to compare premotor time(PMT) and electro-mechanical delay(EMD) between sitting and standing posture. Twenty four healthy young subjects(12 women and 12 men) participated in this study. Subjects were instructed to perform maximal, voluntary, isometric contraction of ankle muscle(tibialis anterior and gastrocnemius muscles) in reaction to auditory stimulus. PMT and EMD, calculated from stimulus, EMG and torque profile were compared between sitting and standing postures. As statistical analysis, paired t-test was performed to assess difference between sitting and standing posture. In both tibialis anterior and gastrocnemius muscles, EMD was found to be significantly longer for standing than sitting. However, PMT in standing posture was longer than that in sitting posture only in gastrocnemius muscles. These result indicate that increased reaction time, particularly, increased EMD of ankle muscles in standing posture may be caused by co-contraction of ankle muscles for postural control in standing posture.