This study was carried out to determine the biological activity of Acanthopanax sessiliflorum fruit extracts. The phenolic compound contents of the extracts were 21.4 and 15.8 mg/g in hot water and 60% ethanol extracts. The total anti-oxidant activities of the water and the 60% ethanol extracts at a 200 ${mu}g/mL$ phenolic concent ration were at $92.4{pm}0.8$ and $89.2{pm}1.1%$ in terms of the DPPH radical scavenging activity, $98.3{pm}1.1$ and $96.5{pm}3.5%$ in terms of the ABTS radical decolorization, $2.0{pm}0.6$ and $1.2{pm}2.8$ PF in terms of the anti-oxidant protection factor, and $66.3{pm}0.8$ and $61.4{pm}2.3%$ in terms of the TBARs inhibitory activity. The activities that inhibited the angiotensin-converting enzyme and xanthin oxidase were at $85.1{pm}3.2$ and 0% in the water extracts and $59.3{pm}1.5$ and $9.5{pm}0.8%$ in the 60% ethanol extracts at the 200 ${mu}g/mL$ phenolic concentration. The tyrosinase and elastase inhibitory activities were at $56.6{pm}1.8$ and $53.1{pm}1.1%$ in the water extracts and $33.7{pm}2.2$ and $22.4{pm}3.1%$ in the 60% ethanol extracts. The astringent effect of the water and the 60% ethanol extracts were at $50.5{pm}0.9$ and $11.5{pm}4.1%$.