The purpose of this study is to examine the effect on R&D investment and technology commercialization capabilities on business performance of 118 Korean firms which has R&D center. In particular, this study tried to define technology commercialization and technology commercialization capabilities for the board and narrow perspective, and investigates the role of technology commercialization capabilities in linking R&D investment and business performance in terms of a financial performance as the growing rate of sales, the growing rate of operating income to sales and non-financial performance as the ratio of technology commercialization. The results of this study are as follows. First, the findings of the research indicated that there was a significant positive relationship between R&D investment and business performance as financial and non-financial. Second, the study found that board technology commercialization capability as technology strategic planning, technology process, and technology organization has the mediating effect to R&D investment on business performance as the ratio of technology commercialization. Third, the study found that narrow technology commercialization capability as manufacturing and marketing play a mediating role in terms of business performance as financial and non-financial.