Simple camera-based system for evaluation of lower limb alignment as a part of an automated cycling fitting system was developed and verified in this study. Developed imaging system can evaluate lower limb alignment quantitatively during pedaling using a general camcorder and single marker attached on the knee. Threshold-based marker detection algorithm was proposed in this study. Experiment was carried out to compare the trajectory data from marker detection algorithm of the developed imaging system with the trajectory data from 3-D motion capture system. Results showed that average error between trajectories was 2.33 mm (0.92 %) in the vertical direction and 0.62 mm (1.86 %) in the medio-lateral direction. There existed significant correlation between two measured values (r=0.9996 in the vertical direction and r=0.9975 in the medio-lateral direction). It can be concluded that developed imaging system be applied to evaluate lower limb alignment which is an important factor for dynamic bicycle fitting.