The purpose of this study was to investigate how induced fatigue of the ankle joint muscles affects the capability and recovery of postural control during single-leg stance in healthy adults. The study population included 22 randomly recruited men and women. Postural control was performed on single-leg stance with eyes open. Ankle joint muscle was fatigued by repeated heel raises. According to the results of this study, for the anteroposterior variables, both men and women showed significantly increased center of mass velocity and decreased center of pressure 95% edge frequency immediately after fatigue. For the mediolateral variables, both men and women showed significantly increased center of mass velocity and decreased center of pressure 95% edge frequency immediately after fatigue. For the total variables, both men and women showed significantly increased center of mass averaged-velocity immediately after fatigue, and also, the center of pressure 95% confidence ellipse area significantly increased in women. Postural control variables were not significantly different for men and women at any time (Pre, P0, P10, and P20). In conclusion, the gender does not affect the capability and recovery after induced fatigue of ankle joint muscles. The effect of fatigue found for the anteroposterior and the mediolateral variables in both men and women. Furthermore ankle joint muscle fatigue led to change of postural control strategy from an ankle joint strategy towards a hip joint strategy. These changes are believed to damage postural control. The ankle joint muscle recovered from fatigue within 20 min during single-leg stance.