In this paper, we proposed a novel location estimation algorithm based on the concept of space-time signature matching in a moving target environment. In contrast to previous fingerprint-based approaches that rely on received signal strength (RSS) information only, the proposed algorithm uses angle, delay, and RSS information from the received signal to form a signature, which in turn is utilized for location estimation. We evaluated the performance of the proposed algorithm in terms of the average probability of error and the average error distance as a function of target movement. Simulation results confirmed the effectiveness of the proposed algorithm for location estimation even in moving target environment.