The main purposes of the agricultural reservoir enlargement (ARE) project are to secure water supply reliability (WSR) for agriculture and to release environmental water during dry seasons. In this study, an operational rule that will simultaneously satisfy both the above issues was developed. Initial amount of water storage at the beginning of non-irrigation season (1st October) was divided into 3 stages, and the target level of water storage at the beginning of irrigation seasons (1st April) was set up. Required operational curves and release amounts were estimated based on the stages and target water levels. To evaluate the applicability of this rule, the amount of water released for environmental purposes and WSRs were analyzed for three reservoirs (Unam, Jangchi and Topjeong). The ratio between annual amount of release and additional amount of water storage were 1.6, 1.85, and 4.1 for the Unam, Jangchi, Tapjeong reservoirs, respectively. Also, the WSRs of all reservoirs were found to become higher than when the design standard was applied. Therefore, it is considered that the proposed rule is more suitable for the enlarged agricultural reservoirs operation as it satisfies the WSRs while securing the environmental water release.