노지 양식장에서 비단잉어와 함께 사육중인 참붕어의 미병부, 체측근, 복부 및 아가미 뚜껑 내부에 황백색의 cyst내의 metacercaria를 탈낭시켜 형태학적 및 분자생물학적으로 조사한 결과 Clinostomum complanatum으로 동정되었다. 병리조직학적으로는 상피세포의 박리와 괴사 및 cyst주변의 근섬유의 위축이외에 다른 조직의 변화는 발견되지 않았다.
Many metacercariae in the musculature of southern top mouthed minnow (Pseudorasbora parva) cultured at a farm in Chonbuk Province. The excysted metacercariae looked yellowish white and were $4.02{pm}1.80$ mm (1.66-5.81) in length and $1.27{pm}0.27$ mm (1.01-1.64) in width for acetocarmin-stained specimens. The oral sucker measuring $0.18{pm}0.05$ mm (0.11-0.24) in length and $0.23{pm}0.07$ mm (0.15-0.33) in width, was located at the anterior end of the body. The ventral sucker situated at the fourth third of the body were measured $0.47{pm}0.08$ mm (0.35-0.53) in length and $0.55{pm}0.09$ mm (0.43-0.63) in width. The tongue-shaped metacercariae were identified as Clinostomum complanatum on the basis of morphological characteristics. The metacercaria was also confirmed to be C. complanatum using PCR reaction with C. complanatum-specific primer sets, Trem 18S F and Ccom670 R. No histopathological changes in the infected fish were observed except necrosis and exfoliation of epidermis in the skin and atrophy of muscle fibers.
