The purpose of the study were to investigate the effect of laughter program on the stress of nursing college students in clinical practice. The research design is one group pretest-posttest survey. Thirty-eight nursing college students took part in the study. Laughter program was carried out for 1hour 6weeks. Laughter program showed significant change on the state anxiety, mood-emotion, laughter index, subjective stress and self esteem. The acquired data were analyzed with SPSS/win 15.0 using descriptive methods paired t-test, Pearson correlation coefficient. The research producted following results: Laughter program can be effective to decrease the clinical practice stress; anxiety, mood-emotion and subjective stress index. Laughter program can be effective to increase laughter index and self esteem of nursing college students. Based on the study, It is necessary to repeat studies and considering to develop proper methods of clinical practice stress of nursing college students. This study recommends that laughter program coupled with cognitive approaches could affect ways of nursing college students coping with stress and contribute to the improvement from increasing their satisfaction.