In this paper, a target signal detection method using multiple signal classification (MUSIC) algorithm is proposed. The MUSIC algorithm is a subspace-based direction of arrival (DOA) estimation method. Using the inverse of the eigenvalue-weighted eigen spectra, the algorithm detects the DOAs of multiple sources. To apply the algorithm in target signal detection for GSC-based beamforming, we utilize its spectral response for the DOA of the target source in noisy conditions. The performance of the proposed target signal detection method is compared with those of the normalized cross-correlation (NCC), the fixed beamforming, and the power ratio method. Experimental results show that the proposed algorithm significantly outperforms the conventional ones in receiver operating characteristics (ROC) curves.