PURPOSES: The aim of this study is to analyze overloading control effectiveness of enforcing overweighted vehicles using HS-WIM (High-Speed Weigh-in-Motion) at main lane of expressway. METHODS: To analyze the weight distribution statistically, HS-WIM system should has an appropriate weighing accuracy. Thus, the weighing accuracy of the two HS-WIM systems was estimated by applying European specifications and ASTM (American Standards for Testing and Materials) for WIM in this study. Based on the results of accuracy test, overweight enforcement system has been operated at main lanes of two expressway routes in order to provide weight informations of overweighted vehicle in real time for enforcement squad. To evaluate the overloading control effectiveness with enforcement, traffic volume and axle loads of trucks for two months at the right after beginning of the enforcement were compared with data set for same periods before the enforcement. RESULTS: As the results of weighing accuracy test, both WIM systems were accepted to the most precise type that can be useful to applicate not only statistical purpose but enforcing on overweight vehicles directly. After the enforcement, the rate of overweighted trucks that weighed over enforcement limits had been decreased by 27% compared with the rate before the enforcement. Especially, the rate of overweighted trucks that weighed over 48 tons had been decreased by 91%. On the other hand, in counterpoint to decrease of the overweighted vehicle, the rate of trucks that weighed under enforcement limits had been increased by 7%. CONCLUSIONS: From the results, it is quite clear that overloading has been controlled since the beginning of the enforcement.