This study identifies the damage detection of truss structures by using genetic algorithm(GA) from changed elements properties. To model the damaged truss structures, the modulus of elasticity of some specific elements is reduced. The analysis of truss structures is performed with static analysis by applying uniform load, and the location and extent of structural damage is detected by comparing the stain of each element of healthy truss structures with damaged truss structures using genetic algorithm. In this study, some numerical examples are presented to detect the location and extent of damage using genetic algorithm.