This paper measures the hot spot temperatures in a single-phase, 154 kV, 15/20 MVA power transformer filled with natural ester fluid using optical fiber sensors and compares them with those calculated by conventional heat run tests. A total of 14 optical fiber sensors were installed on the high-voltage and low-voltage windings to measure the hot spot temperatures. In addition, three thermocouples were installed in the transformer to measure the temperature distribution during the heat run tests. In the low-voltage winding, the hot spot temperature was $108.4^{circ}C$, calculated by the conventional heat run test. However, the hot spot temperature measured using the optical fiber sensor was $129.4^{circ}C$ between turns 2 and 3 on the upper side of the low-voltage winding. Therefore, the hot spot temperature of the low-voltage winding measured using the optical fiber sensor was $21.0^{circ}C$ higher than that calculated by the conventional heat run test.