Objectives This study investigated the relationship between exercise and health factors including vaso-aging degree, stress index, stress resistance, fatigue and activity of autonomic nerve system etc. Methods The subjects were 20,509 persons who had received health examination by Korean Medicine and submitted questionnaires about exercise history, for 8 months from March to November in 2011. The vaso-aging degree were measured by APG (Accelerated Photoplethysmograph). The stress index and resistance, fatigue and activity of autonomic nerve system were measured by HRV (Heart Rate Variability). We analyzed the relationship between exercise and various variables by chi-square test with SPSS ver. 19.0. Results Regarding the relationship between exercise and vaso-aging degree, stress index, stress resistance, fatigue and activity of autonomic nerve system, there were significant differences(p<0.05). Conculsions This study suggests that vaso-aging degree, stress index, stress resistance, fatigue and activity of autonomic nerve system are affected by exercise.