This study was done to determine the seroprevalence of Coxiella burnetii in cattle with reproductive disorders reared in eastern Gyeongbuk province. A total of 146 cattle blood samples from 83 farms in eastern Gyeongbuk province were collected in 2011~2012. Among them, 17 (11.6%) samples from 11 (13.3%) farms were seropositive for C. burnetii by ELISA. In dairy cattle with mastitis, 15 (38.5%) milk samples from 9 (42.9%) farms were seropositive among 39 milk samples from 21 farms. Among the seropositive farms, one farm with high seroprevalence was selected to assess the seroprevalence of C. burnetii in the bulk-tank milk (BTM) and blood samples of dairy cattle. The BTM sample from the farm was seropositive to C. burnetii and 6 (15%) out of 40 dairy cattle were seropositive. In cattle with reproductive disorders including infertility, abortion and stillbirth, 2 (1.9%) samples from 2 (3.2%) farms were positive among 107 samples collected from 62 farms. Among 2 positive samples, one of them is dariy cattle (100%) and the other is Korean cattle (0.9%) (P<0.001). This suggests that Korean cattle was relatively resistant to C. burnetii. Based on the data obtained, we identified association between C. burnetii shedding in milk and chronic subclinical mastitis in dairy cattle. Since seroprevalence of C. burnetii is relatively high in dairy cattle, further intensive studies on C. burnetii among the high risk milk farms and dairy cattle herds are needed to evaluate infection status.