Recently, railroad construction becomes an alternative considering environmental merits such as mass transportation, safety, economy and energy efficiency. Also railroad can have 3 to 4 times better in economy compared to road construction. However, long line and unavoidable sensitive areas hinders railroad construction often resulted in ecosystem degradation. This paper reviewed 27 cases of EIS submitted during 2003-2006 and reviewed major issues on Animal-Plants and Landscape. Noise-Vibration (13.5%), Water Quality (13.5%), Wastes (10.8%) appeared to be most frequent factors to supplement. Route selection in the railroad is the most sensitive issues so that public participation and hearing should be involved in the early stage of the process and social and cultural factors also must be considered to make a final decision on the railroad route location and selection.