Purpose: This prospective study was designed to investigate the prevalence of urinary incontinence (UI), voided volume, post void residual volume (PVRV), daytime frequency and nocturia in women over 40 years. Methods: The sample comprised of 302 women over 40 years.The study lasted from February 2008 to November 2009. Data about daytime frequency and nocturia were gathered from 48 hour bladder diary. Further, a PVRV was assessed through a bladder ultrasonography. Data were analyzed using the t-test, Mann Whitney test, Kruskal Wallis test and multiple regression. Results: Although the prevalence of UI among the women was as high as 70.5%, the perception rate of UI was as low as 10.8%.Single voided volume of women without or with UI was 223 mL or 198 mL (p<.001), respectively; PVRV, 25.8 mL or 23.6 mL (p=.055); daytime frequency, 5.89 or 6.96 (p<.001); nocturia, 0.99 or 1.23 (p=.040). Age (${eta}$=0.19, p=.001), single voided volume (${eta}$=-0.16, p=.006), and nocturia (${eta}$=0.12, p=.034) were associated with UI. Conclusion: The prevalence of UI was found to be 70.5% among the participants. The assessment of single urinary volume and nocturia through a bladder diary lends support to identifying UI for women over 40 years.