This study aimed to investigate the reinforcing effect of two kinds of glass fiber, Quarts Splint$^{TM}$ Mesh and SES MESH$^{(R)}$ and to evaluate the effect of the thermocycling on the transverse strength of the denture base and on the reinforcing effect of the reinforcements. 20 specimens of the size of $2.5{ imes}10.0{ imes}65.0mm$ were fabricated for each group; control group, metal mesh reinforcement group, Quarts Splint$^{TM}$ Mesh reinforcement group and SES MESH$^{(R)}$ reinforcement group. To find the difference made by the thermocycling, 10 specimens of each reinforcement group were treated by thermocycling. 3-point bending test was performed to measure the transverse strength of the denture base resin. The specimens reinforced with SES MESH$^{(R)}$ and Quarts Splint$^{TM}$ Mesh showed significantly higher transverse strength than the control group (P<.05), and significantly lower transverse strength than the specimens reinforced with the metal mesh (P<.05). Thermocycled specimens were lower in transverse strength than non-thermocycled specimens in the control group, metal mesh group, Quarts Splint$^{TM}$ Mesh group and SES MESH$^{(R)}$ group, however significant difference (P<.05) was found only in the control group.