PURPOSE: The purpose of this study was to determine the effects of muscle fatigue on knee joint position sense in the young, elderly adults, and stroke patients. METHODS: The subjects were recruited into three groups; young group(YG)(N=15), elderly group(EG)(N=15), and stroke group(SG)(N=15). Exercise-induced fatigue was achieved by repeated active exercise 60 times on an angle at 10 to 100 degrees of the knee joint with an angle speed of $120^{circ}/s$, with three sets for 15 minutes. Evaluation was performed for determination of repositioning error using passive angle repositioning (PAR) and active angle repositioning (AAR). RESULT: Regarding the error score for the knee joint position sense in both the baseline and muscle fatigue, significant increases were observed between the YG and EG (p<.05), and between the EG and SG (p<.01), and between YG and SG (p<.05) in the PAR, YG and SG (p<.05) in the AAR. CONCLUSION: In particular, the results of this study indicate that management and intervention for elderly adults and stroke patients can result in improvement of proprioception.