The purpose of this paper is to study on the spatial characteristics from the "The Holy Trinity" fresco painted by Masaccio in the early Renaissance paintings. The results of this study are as follows. 1. The elevation composition of the "The Holy Trinity" fresco is divided into the upper and lower structure through horizontal axis on horizontal line around vanishing point. The upper structure is composed of vertical axis formed through the disposition of the "Trinity" elements and horizontal axis on horizontal line. The lower structure is composed of the sarcophagus and skeleton in such inside and the altar supported through circular columns of left and right. 2. The section composition of the "The Holy Trinity" fresco is composed of the ceiling structure of cylindrical-shaped vault on upper part around basic square floor plan that is interior space and upper structure. The exterior space and lower structure is placed with sarcophagus in inner part that altar and step is projected as same height and width in the outside direction. 3. The basic floor composition of the "The Holy Trinity" fresco is planned by square shape around structural columns that is placed at corners as symmetry through transverse, longitudinal and diagonal axis. The whole floor composition planned through the altar and step that is in exterior space at front, the apse of round form at rear part and the structure of the middle story concept at interior. 4. The visual aspect of the "The Holy Trinity" fresco is composed of the stable balance in relation with distance and height because the interior and exterior space as well as the upper structure and lower structure is formed by regular proportion system. The elevation angle of visual range was planned to view in detail generally or partially the architectural composition system and element, characters through proper visual distance, center and position.