본 연구에서는 감귤류 중 온주밀감, 레몬, 한라봉의 과피추출물(PE)에 대한 항염증 효과를 조사하였다. 감귤류 세품종 과피추출물(PE)에 대한 murine macrophage cell line Raw 264.7세포를 배양하여 세포독성, NO생성량, 세포내 염증관련 단백질 발현 양상을 분석하였다. 그 결과 CUP, CHP, CLP 처리구의 경우 2 mg/mL 이하의 농도에서는 세포생육을 저해하지 않았다. LPS (100 ng/mL)를 처리한 세포의 NO생성량을 LPS를 처리하지 않은 대조구와 비교했을 때 상당량이 증가했음을 알 수 있었고, 추출물을 농도별로 처리했을 때 농도가 증가할수록 NO생성량은 감소하였다. 각 처리구에서 각 1 mg/mL 농도에서는 CUP > CHP > CLP 순으로 NO 저해활성이 강하게 나타났다. Western blot의 결과상으로는 CUP와 CHP의 iNOS 단백질 저해활성이 비슷한 강도로 나타남을 확인하였다. COX-2 효소의 저해활성은 CUP > CHP > CLP의 순으로 나타났다. 따라서 CUP, CHP, CLP 처리구는 모두 항염증 작용을 가지고 있으며, 또한 CUP 처리구가 세 개의 추출물 중에서 가장 저해활성이 강하였다.
The following study was presented to investigate the anti-inflammatory effect of peel extracts (PE) from three citrus fruits: Citrus unshiu, Citrus limonia Osbeck and Citrus hallabong. According to this study, cytotoxicity, NO-production and protein levels of iNOS (inducible nitric oxide synthase) in macrophage cell were analyzed, which had been incubated in murine macrophage cell line RAW 264.7 cell of PE from those three citrus fruits. According to Citrus unshiu peel extracts (CUP), Citrus limonia Osbeck peel extracts (CHP) and Citrus hallabong peel extracts (CLP) treatment, the result showed that there was no cell growth inhibited below 2 mg/mL. Comparing the NO-production of the cell with LPS (100 ng/mL) and the treatment without LPS, significant increase of NO-production was detected. However NO-production also showed decrease trend, as the concentration increased. For each treatment, at the concentration of 1 mg/mL, NO-ihibitory activity showed significant result with following order: CUP > CHP > CLP. According to the result from Western blot, the inhibitory activities of iNOS protein from CUP and CHP showed fairly similar performances. Also inhibitory activity of COX-2 showed the following order: CUP > CHP> CLP. There was no doubt that all the treatments of CUP, CHP and CLP have anti-inflammatory effect and also that the inhibitory activity of the CUP treatment was the strongest among those three.
