Objectives : As Daily Dose of Zizyphi Fructus was designated by the numbers in Treatise on Cold Damage Disease, estimation of Daily Dose was all different among researchers. The purpose of this study was to estimate exact Daily Dose of Zizyphi Fructus. Methods : We fixed the errors in various copy of Treatise on Cold Damage Disease and considered the meaning of the Bee Zizyphi Fructus(肥大棗) and general rules of Daily Dose in Treatise on Cold Damage Disease. So we reduced Daily Dose of Zizyphi Fructus, and compared this with the standard of Zizyphy Fructus in Pharmacopoeia of several Nation and Korean Forest Service. Results : Daily Dose of Zizyphi Fructus was generally 12 pieces; less was for prescriptions which scaled down the amount of ingredients prescribed in the originals; 15, 25, and 30 pieces were used when more was required. The medicinal part was the pulp of fructus, and the dosage of 12 pieces was respectively equivalent to 3 Ryang(兩), and 19.5 g. As defined in the Korean Pharmacopoeia Ninth Edition and standards of forest products by Korea Forest Service, Zizyphi Fructus was medium-sized, and weighs about 1.625 g if properly dehydrated. Conclusions : In short, the proper Daily Dose of Zizyphi Fructus in Treatise on Cold damage Disease was 12 pieces of Zizyphi Fructus and 19.5 g of its pulp, weighing three Ryang(兩). The pulp was estimated to be 1.625 g; it was medium-sized according to the present standard.