The purpose of this study was to measure the serum lipid concentrations by gender and age, and to investigate the relationship between dietary behavior and serum lipid levels in Korean adults. The subjects consisted of 209 volunteers (121 men and 88 women) who visited a health promotion center. Their anthropometric and biochemical blood indices were measured, and data on lifestyles were obtained using structured questionnaires. The blood total cholesterol, LDL-cholesterol, and triglyceride concentrations increased significantly with age in women (p<0.01). The food habit score of men and women increased with age, and total food habit score was negatively correlated with BMI (p<0.05). The rate of skipping meals (p<0.001), overeating (p<0.05), eating instant food (p<0.001), and eating out (p<0.05) decreased significantly with age, and the intake rate of vegetables (p<0.001) and carbohydrates (p<0.05) significantly increased with it. The food intake frequency of 21 food items was also measured, and snacks (p<0.05), high fat foods (p<0.01) meats (p<0.05), and instant foods (p<0.01) were negatively correlated with HDL-cholesterol. Snacks (p<0.05) and high fat foods (p<0.01) were positively correlated with LDL-cholesterol. Snacks (p<0.05) and fats/oils (p<0.01) were positively correlated with triglycerides. These results suggest that the food habits and food intake frequency may be one of the important factors affecting serum lipid concentrations in Korean adults.