Purpose: The purpose of the present study was to determine the effects of activation of gluteus maximus (Gmax) and abdominal muscle using EMG biofeedback on lumbosacral and tibiocalcaneal angles in standing position. Methods: Fourteen healthy subjects with normal feet participated in the present study. Electromyographic (EMG) biofeedback using visual cue was used to activate the external oblique (EO) and Gmax. The lumbosacral and tibiocalcalcaneal angles were measured by electronic goniometers. All the subjects were instructed to activate the Gmax and EO monitoring increasing amounts of the muscle activities in each muscle. The lumbosacral and tibiocalcaneal angles were collected in three trials during resting and activation of each muscle using EMG biofeedback in standing position. The mean value of three trials was used in the data analysis. A paired-t test was used to compare the lumbosacral and tibiocalcaneal angles between resting and activation of the Gmax and EO using EMG biofeedback. Results: The lumbosacral and tibiocalcaneal angles were significantly less in the resting compared to activation using EMG biofeedback (p<0.05). Conclusion: The activaition of Gmax and abdominal muscles using EMG biofeedback play role to control the pronation of subtalar joint during the weight-bearing.