The aim of this study was to investigate the difference of electrodermal activity between the hypertensive and non-hypertensive subjects. A total 161 subjects who visited a hospital for health check ups from August 2011 through November 2011 and from September 2012 through November 2012 were included in this study. We divided the subjects into two groups by blood pressure, hypertension group(81 patients) and non-hypertension group(180 subject). Then we compared electrodermal activity between two groups by using the autonomic bioelectric response device model CP-6000A. The data was analyzed by independent t-test and logistic regression. In this study, there was a significant difference in electrodermal activity(the mean absolute value of positive, negative pulse conductivity) between hypertensive and normotensive group in t-test and electrodermal activity showed the significant influence on the prevalence risk of hypertension with age, body mass index and dyslipidema in binominal logistic regression. In conclusion, hypertensive patients had high level of electrodermal activity compared with normotensive subjects and this finding suggests that electrodermal activity which is explained excess tendency in Korean traditional medicine could be used for diagnosis in hypertension patients at Korean traditional medical clinic.