The impact and spreading behaviors of silicon dioxide nanoparticle colloidal suspension droplets were quantitatively visualized using a high-speed imaging system. Millimeter-scale droplets were generated by a syringe pump and a needle. Droplets of different velocity were impacted on a non-porous solid surface. Images were consecutively recorded using a CMOS high-speed camera at 5000 fps (frames per second) for millimeter-scale droplets. Temporal variations of droplet diameter, velocity and maximum spreading diameters were evaluated from the sequential images captured for each experimental condition. Effects of Reynolds number, Weber number, and particle concentration were investigated experimentally.