Objective : To clarify the features of needling method in the Zhenjiu Jiayijing, the needling depth and needling treatment time were sorted out. Method : According to the analysis, we can identify the crucial factors of the needling features. Result : Compared with the needling related content of Huangdi Neijing(?帝??), the historical outline of the needling development could be found and the ancient acupuncturing process was reappeared with a historical perspective. This research will provide guidance for the literature research and clinical application. Conclusion : Zhenjiu Jiayijing, also an important and valuable literature, will provide more evidence or clues for depth and treatment time of needling, which will be helpful and meaningful in clinic.