Bone is so crucial anatomy for human body. Many researchers study deep into a subject about bone regeneration. There is no standard analysis for quantitative Neo-bone regeneration on calvarial defected model. Micro CT is so useful method to quantitative analysis of Neo-bone regeneration. This study was show that how to quantitative analysis of Neo-bone regeneration with ${mu}-CT$ Micro CT was possible to quantitative analysis for Neo-bone regeneration on Calvarial defected model. futhermore Not only was Micro CT possible for qualitative analysis but quantitative analysis on the mouse calvarial model. This study will provide bone biology researchers with accurate quantitative analysis.