Distortional and local buckling are important factors that influences the bearing capacity of steel-concrete composite box-beam. Through theoretical analysis of distortional buckling forms, a stability analysis calculation model of composite box beam considering rotation of steel beam top flange is presented. The critical bending moment calculation formula of distortional buckling is established. In addition, mechanical behaviors of a steel beam web in the negative moment zone subjected separately to bending stress, shear stress and combined stress are investigated. Elastic buckling factors of steel web under different stress conditions are calculated. On the basis of local buckling analysis results, a limiting value for height-to thickness ratio of a steel web in the elastic stage is proposed. Numerical examples are presented to verify the proposed models.