Prestressed concrete (PC) is the predominant material in highway bridge construction. The use of high-strength concrete has gained wide acceptance in the PC industry. The main target in the highway industry is to increase the durability and the life-span of bridges. Cracking of elements is one aspect which affects durability. Recently, nine 7.62 meter long PC I-beams made with different concrete strength were designed according to a simple, semi-empirical equation developed at the University of Houston (UH) (Laskar et al., ACI Journal 107(3): 330-339, 2010). The UH Method is a function of shear span-to-depth ratio (a/d), concrete strength $sqrt{f^{prime}_c}$, web area $b_wd$, and amount of transverse steel. Based on testing these girders, the shear cracking strength of girders with different concrete strength and different shear span-to-depth ratio was investigated and compared to the available approaches in current codes such as ACI 318-11 (2011) and AASHTO LRFD Specifications (2010).