The aerodynamic characteristics (subglottal pressure (Ps) and mean airflow rate (MFR)), fundamental frequency (Fo), intensity (I), vocal efficiency (VE), and closed quotient (CQ) were compared during a sustained vowel /o/ sound under three conditions: in a comfortable loudness and pitch level (condition 1), in a maximum loudness level with a fixed pitch (condition 2), and in a maximum loudness level without a fixed pitch (condition 3). Also, multiple regression analyses were done to measure the aerodynamic characteristics affect on the VE and the CQ in each condition. The results showed the Fo, Ps, MFR, VE, and CQ increased as I increased with and without fixed pitch. Most notably, VE in condition 3 was the highest of all the conditions, but CQ was not very high. By the results of multiple regression analysis, VE was significantly affected by I and Ps in all conditions; Fo was the other main key for affecting VE in high pitch. However, none of the aerodynamic characteristics significantly affected CQ. As I increases, Fo should be increased by increasing Ps and VE. Therefore, researchers should consider and specify an a priori to Fo, Ps, and I when measuring VE to examine the complex and delicate vocal mechanism.