Objectives: This report aimed to demonstrate effect of human placental extract (HPE) pharmacopuncture in students suffering fatigue due to hard work and mental stress. Methods: Thirty-four college students, preparing for the final-term exam, received pharmacopuncture of HPE into Kwanwon (CV4) subcutaneously twice a week for 7 weeks. After every pharmacopuncture of HPE, degree of fatigue was surveyed by questionnaire including physical condition, physical endurance, concentration and memory. Results: Pharmacopuncture of HPE into CV4 gently improved physical condition, physical endurance, concentration and memory. Improvement of physical condition was especially positively correlated with the increasing number of pharmacopuncture of HPE into CV4 as confirmed by calculation of Pearson coefficient. Conclusions: Pharmacopuncture of HPE into CV4 alleviated fatigue in the students from hard work and mental stress.