The fruits of Pourthiaea villosa were extracted with methanol and its extract was fractionated with n-hexane, methylene chloride, ethyl acetate and n-butanol. Repeated column chromatography of silica gel, sephadex LH-20 and HPLC led to the isolation of nine phenolic compounds from ethyl acetate soluble fraction. The chemical structures were elucidated as kaemferol-3-O-${eta}$-D-glucopyranoside (astragalin) (1), isorhamnetin-3-O-${eta}$-D-glucopyranoside (2), kaempferol-3-O-${eta}$-D-xylopyranosyl($1{ ightarrow}2$)-${alpha}$-L-rhamnopyranoside (3), caffeic acid (4), quercetin-3-O-${eta}$-D-xylopyranosyl($1{ ightarrow}2$)-a-L-rhamnopyranoside (5), quercetin-3-O-${eta}$-D-xylopyranosyl($1{ ightarrow}2$)-${eta}$-D-glucopyranoside (6), quercetin-3-O-${eta}$-D-xylopyranosyl($1{ ightarrow}2$)-${eta}$-D-galactopyranoside (7), quercetin-3-O-${alpha}$-L-rhamnopyranoside (quercitrin) (8), and kaempferol-3-O-${alpha}$-L-rhamnopyranoside (afzelin) (9) by spectroscopic techniques. These compounds were isolated from this plant for the first time.