Objective: The purpose of this study was to analyze the effects of wearing spandex underwear on the muscle activity and heart rate during flexion and extension of trunk and knee joint. Background: Back pain continues to be the leading overall cause of physical illness and lowering productivity in workplace. Recently, the utility of assistive garment or back belt have been discussed to lessen the back pain as well as to preserve the right posture. Method: Ten males and ten females participated in the experiment. Gender, wearing spandex underwear and postures were used for independent variables, and muscle activity, the level of asymmetry of muscle activity and variation of heart rate were used as dependent variables. Muscle activity and heart rate were measured during flexion and extension of trunk and knee joint. Results: When wearing spandex underwear, the muscle activity was relatively low compared to the condition not wearing the spandex underwear. The symmetry of muscle activity and the variation of heart rate were statistically significant at the 0.05 significance level. In particular, it was found that the symmetry of muscle activity increased while wearing spandex underwear. Conclusion: Spandex underwear has an effect that can reduce asymmetry of right and left muscles which are relevant to leg because spandex underwear equally supports right and left low-back muscles. Application: The spandex underwear used in this study can apply to the rehabilitation as an useful assistive device.