Tissue equivalent proportional counter (TEPC) can measure the Linear Energy Transfer (LET) spectrum and calculate the equivalent dose for the complicated radiation field in space. In this paper, we developed and characterized a TEPC for radiation monitoring in International Space Station (ISS). The prototype TEPC which can simulate a 2 ${mu}m$ of the site diameter for micro-dosimetry has been tested with a standard alpha source ($^{241}Am$, 5.5 MeV). Also, the calibration of the TEPC was performed by the $^{252}Cf$ neutron standard source in Korea Research Institute of Standards and Science (KRISS). The determined calibration factor was $k_f=3.59{ imes}10^{-7}$ mSv/R.