Antimicrobial activities of the five fractions obtained from the methanol extract of Sebasticania corniculata plant were evaluated against five food poisoning bacteria using the agar diffusion method. The chloroform fraction possessed strong antimicrobial activities against five food poisoning bacteria. 8-Hydroxyquinoline was isolated from the chloroform fraction by the various chromatography analyses. When to the agar diffusion method was used, 8-hydroxyquinoline showed potent antimicrobial activities against five food poisoning bacteria. In the case of minimum bactericidal concentration or minimum inhibitory concentration, 8-hydroxyquinoline showed significantly higher antimicrobial activity against five food poisoning bacteria. Thus, the extract of S. corniculata and 8-hydroxyquinoline could be useful for the development of eco-friendly food supplemental agents.