${alpha}$-amylase and pectinase showed good compatibility. A desizing ratio of 95.4 %, a pectin removal rate of 80.4 % and a capillary effect height of 7.1 cm for cotton fabrics treated with ${alpha}$-amylase and pectinase were obtained by a one-bath for bio-desizing and bio-scouring process under the condition of $90^{circ}C$ for 30 min. After the treatment of the nonionic surfactant Peregal O at $100^{circ}C$ for 20 min, these important properties for the cotton fabrics were further improved to 98.7 %, 96.8 %, and 18.4 cm separately. The capillary effect height of desized cotton fabrics was improved from 0.2 cm to 6.4 cm by the removal of waxes because its hydrophobic nature of the cotton fabrics. The whole time for this new combining process with high temperature treatment was significantly shortened and it only took about 55 minutes.