In this paper, the biological removal of $H_2S$ from air had been investigated using a self-made biofilter with efficient bioceramics and a polyhedral hollow ball. The biological removal efficiency of $H_2S$ had been analyzed at different experimental conditions, such as inlet $H_2S$ concentration, residence time, initial pH value, and reaction temperature etc. The results showed that the initial pH value had a slight effect on $H_2S$ removal efficiency from pH 3 to 9. The optimal initial pH value was 5.5, while the $H_2S$ removal efficiency was 100%. The $H_2S$ removal efficiency increased with increases in the nutrient solution spraying rate. The appropriate temperature was $25^{circ}C$ in the temperature range from 15 to $30^{circ}C$. The $H_2S$ removal efficiency dropped with the increase of air input and inlet $H_2S$ concentration. After being isolated and screened, six strains of heterotrophic sulfide oxidizing bacteria and one strain autotrophic sulfide oxidizing bacteria were determined to be involved in the removal of $H_2S$ within the biofilter. The reaction kinetics of $H_2S$ was in accordance with first order reaction kinetics.